ABSTRACT
ObjectiveTo investigate the effects of Buyang Huanwutang （BYWHT） on reducing inflammatory injury and improving neurological function after cerebral ischemia-reperfusion in rats via activating the adiponectin （APN） pathway. MethodMale SD rats were randomized into sham， model， BYHWT （16 g·kg-1， twice a day）， and BYHWT + APN inhibitor （GW9662） groups. In the sham group， blood vessels were isolated. The rat model of middle cerebral artery occlusion （MCAO） was established. The rats in the BYHWT+GW9662 group was treated with subcutaneous injection of GW9662 at 4 m·kg-1 30 min before MCAO surgery and BYHWT at 16 g·kg-1 by gavage after MCAO surgery， once in the morning and once in the evening. The immunofluorescence （IF） assay was employed to observe the expression of adiponectin receptor 1 （AdipoR1） and the colocalization of AdipoR1 with neuronal nuclei （NeuN） and ionized calcium binding adaptor molecule 1 （Iba1） in the brain. Enzyme-linked immunosorbent assay （ELISA） was employed to detect the expression of APN in the serum and brain. The balance beam test was carried out to examine the balance ability， and the grasping test to assess the recovery of limb strength. The immunofluorescence assay was used to detect the expression of myeloperoxidase （MPO）. Western blot was employed to determine the expression of tumor necrosis factor-alpha （TNF-α）， interleukin （IL）-1β， IL-6， and IL-10 and in the brain. ResultCompared with the sham group， the modeling promoted the expression of AdipoR1 （P<0.01）， lowered the APN levels in the serum and brain （P<0.05， P<0.01）， increased the score in the balance beam test （P<0.01）， and decreased the grasping strength of forepaw （P<0.01）， which were accompanied with increased MPO， TNF-α， IL-1β， and IL-6 levels （P<0.01） and decreased IL-10 level （P<0.01）. Compared with the model group， BYHWT promoted the expression of AdipoR1 （P<0.01）， elevated APN levels in the serum and brain （P<0.05， P<0.01）， and increased the grasping strength of forepaw （P<0.01）， which were accompanied with lowered MPO， TNF-α， IL-1β， and IL-6 levels （P<0.01） and elevated IL-10 level （P<0.01）. All the above effects were partially blocked by GW9662. ConclusionBYHWT can reduce inflammatory injury and improve neurological function in the rat model of cerebral ischemia-reperfusion by activating the APN pathway.
ABSTRACT
Objective To explore the efficacy of targeted care for sleep by analyzing polysomnography (PSG) of the different stages of night sleep in patients with depressive disorder. Methods 80 depressive patients met the recruited criteria from Feb 2015 to Feb 2016 were sample-randomly divided into research group with targeted care and controlled group with routine nurse by random number table. PSG of each case was analyzed and Hamilton Depression Scale (HAMD) and Self-Rating Depression Scale (SDS) were used to assess at baseline and endpoint during 4 weeks period. Results Total sleep time, sleep efficiency, N3 and rapid eyes movement ratio of PSG were (395.74 ± 29.31) min, (80.42±1.92)%, (19.11±2.29)%and (16.01±2.55)%in research group, (372.34±24.37) min, (72.61 ± 1.64)%, (13.61 ± 2.38)%and (13.87 ± 2.03)%in controlled group (t=2.892-13.361, P<0.05). SDS at 2nd week and 4th week were (45.28 ± 6.28) points and (32.62 ± 4.65) points in research group, (48.63±7.54) points and (36.32±4.34) points in controlled group (t=-4.12,-4.43, P<0.05). HAMD at 2nd week and 4th week were (15.92 ± 2.62) points, (9.94 ± 2.32) points in research group, (18.27 ± 3.41) points and (12.45 ± 2.21) points in controlled group (t=-3.51,-4.35, P<0.05). Conclusions Efficacy of targeted care for sleep in patients with depressive disorder is better and sooner, and the intervention is good for both sleep quality and mood level.